Showing posts with label RT-PCR. Show all posts
Showing posts with label RT-PCR. Show all posts
Sunday, 3 April 2016

Usefulness of urine samples for Zika virus testing...

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There is a narrow window of Zika virus (ZIKV) detectability in the blood (viraemia) based on the RT-PCR testing of serum samples from sympto...
Sunday, 6 March 2016

Detecting Zika viruses using PCR: a look at published assays...

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[NOTE: I'll update this post with other assays and information as I find time] You may be helped - hopefully anyway - by reading the ...
Friday, 29 January 2016

Zika virus disease samples...don't pass urine (by)..

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It is becoming anecdotal in the Tweet'verse that Zika virus (ZIKV) viraemia (virus in the blood) can only be detected for 5 days - presu...
Friday, 23 October 2015

Markets that deal in camels may help spread MERS-CoV variants..

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This camel/MERS-CoV study from Farag and colleagues, serves as follow-up of sorts to my last post . The paper, which was published in July 2...
Sunday, 18 October 2015

A negative viral culture result is not the end of the story...just a negative result

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Lately, for both Ebola virus and Middle East respiratory syndrome coronavirus, there have been instances where I've been reminded that o...
Tuesday, 5 May 2015

PCR primers...a primer!

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This post has been moved to the new  Virology Down Under  platform on Wordpress. You can get to this specific post by clicking on the link b...

Reverse transcription polymerase chain reaction (RT-PCR)...a primer

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This post has been moved to the new Virology Down Under platform on Wordpress. You can get to this specific post by clicking on the link ...
Friday, 16 January 2015

MERS-CoV snapdate on canaries...

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MERS-CoV detections among healthcare workers (HCWs) HCWs are akin to the canary in the coal mine - when HCWs get sick with a particula...
Monday, 17 November 2014

Ebola testing: 48-72 hours for a negative to turn positive

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Currently, some fraction of the people who present very early after they may have been infected by Ebola virus for testing, return a negat...
Thursday, 12 June 2014

MERS-CoV in the blood....

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The Middle East respiratory syndrome coronavirus (MERS-CoV) is, at its core, a respiratory virus. Well, as far as we know it is anyway. Bu...
Thursday, 23 January 2014

Market sampling: H7N9, sensitive testing, market closures and small numbers

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A World Health Organization Western Pacific Region update on influenza A (H7N9) virus has a few interesting bits of information that pulls t...
Monday, 13 January 2014

H5N1 case in Canada had been diagnosed with pneumonia...testing at the source would have been helpful

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And now, from a fantastically detailed post onto ProMED by Fonseca and colleagues, we see that the H5N1 case was diagnosed with pneumonia. ...
Tuesday, 31 December 2013

Qatari camels clear coronavirus

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The camel herd that was previously Middle East respiratory syndrome coronavirus (MES-CoV) RT-PCR-positive is no longer positive for viral ...
Monday, 25 November 2013

No symptoms but still shedding virus?

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Click on image to enlarge. A stylized trace of the temperatures during a PCR cycle. D-denaturation, when primers and double-stranded D...